RE: Mouse tissue processing protocol

From:"Monson, Frederick C."

Hi Renee,
	The problem you describe, in my experience, is almost always caused
by incomplete dehydration and/or clearing, or excessive heat during
infiltration.  Once I added a 70% ethanol step of 24 hours and a vacuum
infiltration step after the paraffin baths on my AutoTechnicon, the problem
went away.  I was particularly pleased to find, later in life, that fixation
with my own formaldehyde fixative (no longer from Formalin but from 20%
formaldehyde from paraformaldehyde) and always buffered, that everything
seemed to work better.

Hope the same is true for you,

Fred Monson

Frederick C. Monson, PhD
The best research
Center for Advanced Scientific Imaging
occurs before work
West Chester University
at the bench.
West Chester, Pennsylvania, USA, 19383

> ----------
> From: 	Renee Hoyle-Thacker
> Sent: 	Wednesday, January 16, 2002 2:46 PM
> To:
> Subject: 	Mouse tissue processing protocol
> Hello everyone,
> I was wondering if research people would be willing to share their 
> processing schedule particularly their mouse tissue protocol.  We have had
> trouble with dry liver, spleen, sternum, etc.  At times we must soak
> blocks 
> for 1 hr or more and still obtain dry sections, we think the problem might
> be over-processing.  Thanks for your help!
> Renee
> Renee Hoyle-Thacker
> CIIT Centers for Health Research
> 6 Davis Dr.
> Research Triangle Park NC 27709
> Voice: (919) 558-1322

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