RE: GMS help
Someone privately asked me how I dispose of my chromic acid. I felt this
would be better to share with everyone whilst we're on the topic.
I re- use my chromic acid 2 to 3 times in staining (I figure about 6 slides
worth), then I pour it into the pot with my glass cleaning solution and add
a sufficient amount of H2SO4. I don't do a lot of GMS or Gridley's --- or
any other special stain for that matter --- so I don't generate a lot of
chromic acid waste. However, it eventually gets taken away by our
Environmental Health and Safety dept.
Gayle C. I agree completely with your comments. However, being the
Curious George that I am, I have to try this. We don't have Histoplasma
here in Utah, so this isn't such a problem for us, but ... if it were
present and missed, I can only imagine the litigation that would ensue.
gotta run folks
At 08:59 AM 1/16/02 -0700, Connie McManus wrote:
>Do you prepare your own reagents in-house, or do you buy themcommercially?
>If commercially, who do you get your kit from?
>I continue to use the 4% chromic acid in my GMS. I have not heardof using
>Periodic acid as an oxidser for GMS, but I think I'll try it side by side
>the usual sometime to compare the two. I'm curious to see if there are any
>At 08:41 AM 1/16/02 -0600, Johnson, Teri wrote:
>> I have no cited literature on this, only a technique provided by
>>another Histology laboratory that was using it. Teri Johnson
>>Manager Histology Core Facility
>>Stowers Institute for Medical Research
>>1000 E. 50th St.
>>firstname.lastname@example.org -----Original Message-----
>>From: Noreen Gilman [mailto:Ngilman@nbhd.org]
>>Sent: Monday, January 14, 2002 9:53 AM
>>Subject: GMS help
>> and I had never heard of this. They are going to fax me a copy, but
>>I thought I'd get some feedback from you guys first. TIA
>>Noreen Noreen S. Gilman, HT (ASCP) CLS
>>Broward General Medical Center
>>Ft. Lauderdale, FL 3316
>Veterinary Diagnostics Lab
>Utah State University
>fax (435) 797-2805
Veterinary Diagnostics Lab
Utah State University
fax (435) 797-2805
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