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At the clinical lab I worked in previously, we moved away from chromic acid because, as Dr. Richmond indicated, we wanted to eliminate its use in our laboratory.  We substituted 4% Periodic Acid for the 4% chromic acid with no apparent deleterious effect.  Using this oxidation combined with an ammoniacal silver solution in the microwave, we were able to achieve sufficient impregnation, including Histoplasma organisms.  I have no cited literature on this, only a technique provided by another Histology laboratory that was using it. 
 
Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, Missouri  64110 
tjj@stowers-institute.org 

-----Original Message-----
From: Noreen Gilman [mailto:Ngilman@nbhd.org]
Sent: Monday, January 14, 2002 9:53 AM
To: histonet@pathology.swmed.edu
Subject: GMS help


Once again I find myself asking for special stain help from my fellow histonetters. This concerns the GMS stain. One of my techs tells me that a friend of hers from another hospital is using Periodic Acid instead of Chromic acid, and that there is a written procedure for it. I must admit that I have been out of the technical area for several years, and I had never heard of this. They are going to fax me a copy, but I thought I'd get some feedback from you guys first.
TIA
Noreen
 
Noreen S. Gilman, HT (ASCP) CLS
Supervisor Histopathology
Broward General Medical Center
Ft. Lauderdale, FL 3316
954-355-5592 Phone
954-355-4139 Fax
ngilman@nbhd.org


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At the clinical lab I worked in previously, 
we moved away from chromic acid because, as Dr. Richmond indicated, we 
wanted to eliminate its use in our laboratory.  We substituted 4% Periodic 
Acid for the 4% chromic acid with no apparent deleterious effect.  Using 
this oxidation combined with an ammoniacal silver solution in the microwave, we 
were able to achieve sufficient impregnation, including Histoplasma 
organisms.  I have no cited literature on this, only a technique provided 
by another Histology laboratory that was using it. 
 

Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, 
Missouri  64110 
tjj@stowers-institute.org 

  
-----Original Message-----
From: Noreen Gilman 
  [mailto:Ngilman@nbhd.org]
Sent: Monday, January 14, 2002 9:53 
  AM
To: histonet@pathology.swmed.edu
Subject: GMS 
  help


  
Once again I find myself asking for special stain help from my fellow 
  histonetters. This concerns the GMS stain. One of my techs tells me that a 
  friend of hers from another hospital is using Periodic Acid instead of Chromic 
  acid, and that there is a written procedure for it. I must admit that I have 
  been out of the technical area for several years, and I had never heard 
  of this. They are going to fax me a copy, but I thought I'd get some feedback 
  from you guys first.
  
TIA
Noreen
  
 
  
Noreen S. Gilman, HT (ASCP) CLS
Supervisor Histopathology
Broward 
  General Medical Center
Ft. Lauderdale, FL 3316
954-355-5592 
  Phone
954-355-4139 Fax
ngilman@nbhd.org

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