|From:||"Bright, Alan" |
It seems that your microtome chamber temperature is too warm try minus 28 degrees C, all your troubles will be over.
Bright Instrument Co.Ltd.
St Margaret's Way
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site: www.brightinstruments.com
From: April E. Monchik [mailto:firstname.lastname@example.org]
Sent: 29 January 2002 16:07
Subject: Daily Digest
I work in a Histology Core Facility at Cornell Medical School as a technician. I have been asked to freeze and section wounded mouse skin. The pieces are 1inch x 0.5 inches. There have been problems with the tissue ripping away from the Tissue Tek when I try to section it. The skin is so soft that it also seem to fold on itself while freezing. Does anyone have suggestions for frozen sectioning of skin?
I appreciate any insight you may have