Dear April,

It seems that your microtome chamber temperature is too warm try minus 28 degrees C, all your troubles will be over.

Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright@brightinstruments.com
Web Site: www.brightinstruments.com
-----Original Message-----
From: April E. Monchik [mailto:aem2002@med.cornell.edu]
Sent: 29 January 2002 16:07
To: HistoNet@pathology.swmed.edu
Subject: Daily Digest

Hi,
I work in a Histology Core Facility at Cornell Medical School as a technician.  I have been asked to freeze and section wounded mouse skin.  The pieces are 1inch x 0.5 inches.  There have been problems with the tissue ripping away from the Tissue Tek when I try to section it.  The skin is so soft that it also seem to fold on itself while freezing.  Does anyone have suggestions for frozen sectioning of skin?

I appreciate any insight you may have
Thanks
April