We use squares cut from plastic autoclave bags. That is tedious, but cheap
and it works well.

Tim Morken
CDC, Atlanta



-----Original Message-----
From: Kimberly L Merriam [mailto:kmerriam@tktx.com]
Sent: Wednesday, January 23, 2002 4:45 PM
To: Histonet
Subject: Coverslips/Gaskets for ISH


Hi,

I will be starting some in situ hybridization (DNA probe) in
the near future and am looking for some advice on types of
coverslips and/or gaskets to use while incubating with the
probe.  EMS has several different types (Probe-Clip,
CoverWell, Hybriwell, Hybrislip, Perfusion Chambers and
Press-to-seal Silicone Isolators).  Any opinions,
advantages, disadvantages on them would be appreciated.  I
don't know which one to try!

Thanks in advance,
Kim Merriam
TKT
Cambridge, MA