Paraffin processing brain slices
Hi Bert, We standardly paraffin process 3mm thick sections of rat brain
that have been dropped fixed in 10% NBF or perfusion fixed by NBF or
GLUT/PARAF. I have cut 5 to 30 microns paraffin sections with no problems
except that a longer soaking time is required for sections over 10 microns.
Our rat tissue processing schedule is about 8 hours starting with 70 %
ETOH. The brain tissue does have to be adequately fixed. We also cut 60
micron frozen sections from NBFF brains that have been frozen on chucks.
CIIT Centers for Health Research
PO Box 12137
Research Triangle Park, NC 27709
Voice Mail-(919) 558-1252
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