Paraffin processing brain slices

From:Delorise Williams

Hi Bert, We standardly paraffin process 3mm thick sections of rat brain 
that have been dropped fixed in 10% NBF or perfusion fixed by NBF or 
GLUT/PARAF. I have cut 5 to 30 microns paraffin sections with no problems 
except that a longer soaking time is required for sections over 10 microns. 
Our rat tissue processing schedule is about 8 hours starting with 70 % 
ETOH. The brain tissue does have to be adequately fixed. We also cut 60 
micron frozen sections from NBFF brains that have been frozen on chucks.

Delorise Williams
CIIT Centers for Health Research
PO Box 12137
Research Triangle Park, NC  27709
(919) 558-1200	
Voice Mail-(919) 558-1252
Fax-(919) 558-1300

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