Immunos on Mouse tissue

I am in need of some help? I am trying to do immunos on mouse tissue. I
currently use the Dako LSAB+ kit and I know that some of my antibodies are
monoclonal. I have tried the Dako ARK kit and have not had any luck. I don't
know what I may have done wrong. I also have tried the Dako Envision
polyclonal kit. I have changed my antibodies from mono to poly or goat. I
have tried different retrieval methods. What can I do now? Any help would be
appreciated. The two antibodies I am working with mostly is the Cd31 (goat
polyclonal and Rat anti mouse) and Collagen 4 (monoclonal and polyclonal).
Donella Stillings
University of Colorado HSC

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