Fwd: Re: "Overprocessed tissue."
|From:||"Dr. Ian Montgomery" |
Cedarwood oil from Merck in the UK but I'm sure there must be a US
supplier. As for a schedule the best place is John Kiernan's book,
Histological and Histochemical Methods. Chapter 4, Processing and
mounting, gives details of clearing agents and suitable schedules.
John's book is one of these texts everyone should have, a mine of solid
gold nuggets. Cedarwood oil is a bit expensive so you'd have to weigh the
benefits before using heavily. I routinely use chloroform overnight for
clearing with a final 15 minutes in xylene as the link agent with wax. If
it's a tissue I know will be difficult then it gets cedarwood oil as the
Dr. Ian Montgomery,
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Date: Wed, 23 Jan 2002 15:53:01 -0700
To: "Dr. Ian Montgomery"
From: Connie McManus <firstname.lastname@example.org>
Subject: Re: "Overprocessed tissue."
I didn't know anyone still used cedarwood oil for clearing tissues!
know of a US supplier? I have only heard of using this, never
experienced it. I would love to try this... if it is
feasable. I want to try it anyway. Would you care to share
much thanks from across the Pond
Connie McManus in Utah
At 01:40 PM 1/23/02 +0000, you wrote:
>> Recently there has been series of
postings regarding overprocessed
tissue that have left me totally confused. The object in processing
embedding in paraffin wax is the complete removal of water from tissue
it's replacement by wax. Therefore if overprocessing has occurred
has been lost and replaced by wax. If that is what's meant then the
overprocessed tissue is only fit for the rubbish bin. I really think
the term overprocessing should be dropped and replaced by something
suitable and probably more accurate such as under processed,
it wasn't me it was the machine and so forth. From the descriptions
give I think water is still present in the tissue or users have moved
from "proper" clearing agents and rely on xylene or a
substitute as the
agent of choice which can result in "crumbly" blocks. I still
chloroform, cedarwood oil etc., "real" clearing agents and when
has been thoroughly dehydrated, cleared and embedded the resulting
are a dream.
> Then we have the next wee
problem. "How long did you fix the
tissue." "Oh, not very long, it's only been in Bouin for a
months. That's alright, isn't it."
Veterinary Diagnostics Lab
Utah State University
fax (435) 797-2805
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 332 8855 Extn.6644.
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