Eosinophils and Mast cells.

From:"Dr. Ian Montgomery"

        Starting a small pilot project looking at eosinophil and mast cell distribution, so in the first instance I'll restrict myself to conventional techniques. Carbol chromotrope (Lendrum 1944) for the eosinophils, simple and very reliable. Mast cells, well, mmmm, so many choices but probably Methylene blue (Unna), Toluidine blue or Thionin (Cook 1961).
        So, any suggestions for something better for the mast cells and what about a technique that will demonstrate both cell types.  Remember it's only a pilot, so if possible I don't want to buy expensive antibodies that might only be used a few times. 

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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