Double labeling for IFC

From:Jasmine Piep

Hi all,

I have a quick question about double labeling for IFC.

I have been using single labeling for two different antibody and both worked 
fine. However, I use two different block solution to block each of my 
primary antibody. One is block with anti-mouse in 1% BSA/TBST for my Mono. 
Mouse antibody and the other is block with 1% normal goat serum in 1% 
BSA/TBST for Mono. Rabbit antibody.

My question is I want to do double labeling for both of my antibody, but 
STICK with what to do with the blocking step. The tissue is Skin and Liver.

Many thanks if anyone can help.
Jasmine





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