Double labeling BrdU/Ki-67 in rabbit

From:Patrick Ladage



Please advise: I would like to double label BrdU/Ki-67 in the rabbit cornea, preferably en bloc and otherwise in cryostat sections. 

 (1)  Is there a Ki-67 antibody that works in the rabbit and that is not a mouse monoclonal?

(2)  What about BrdU? I tried sheep polyclonal but it gives too much nonspecific background staining.

(3)  Do you have some references on double labeling BrdU/Ki-67 in the rabbit.

 Thanks for your help,




Patrick M Ladage B.Optom F.A.A.O.
Jr. Postdoctoral researcher
Department of Ophthalmology
UT Southwestern Medical Center, Dallas, TX
5323 Harry Hines blvd, Dallas, TX 75390-9057
(P) 214- 648- 9544
(F) 214- 648- 8447

Ph.D. candidate
College of Optometry
University of Houston, Houston, Texas

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