: Technovit 7200
Yes, I have a stain for VVG on Technovit 7200.
1. Etch section for 2 minutes in 1% formic acid (Hydrochloric will cause
the Technovit 7200 to crack).
2. Then I use the Verhoeff's kit from Poly Scientific (cat. # K059) and
start with step 3 with immersion in Verhoeff's working solution and go all
the way through Van Gieson's solution. Do not clear in Xylene or
coverslip. Store slides in the dark and take pictures as soon as possible
using immersion oil and a temporary coverslip for high magnification.
I hesitate to give you the times that I used as this stain has to be
closely monitored under the microscope at every step. The times given in
most procedures are for paraffin embedded sections. With the Technovit
7200 plastic sections, there are many factors that come into play. A lot
depends on the thickness of the ground section. Thinner sections usually
require longer staining times. Best advice is to start with 1 - 5 minute
increments and increase accordingly- checking closely with the
microscope. In other words, "tweak, tweak, tweak"! :-) -- The motto for
plastic section staining!
Linda Jenkins, HT
<< Previous Message | Next Message >>