Hello Histonet-ters;

I wonder if you could help me with the following:

I am looking at "muscle biopsies" from volunteers (mice) who have received
focused ultrasound treatment.  Of course enough excess heat would cook any
tissue, but I we are trying to detect much more subtle changes/damage
produced by a lot less heat.  

I know TTC has been used on hearts to detect ischemic necrosis associated
with myocardial infarction, but as I understand it, you need to soak the
tissue *before* paraffin embedding, and TTC is intended to show areas of
necrosis at the gross, not microscopic level.

Is there anything out there for the microscopic level that would highlight
areas of damage?

Thanks for all your help.

Judit Zubovits