Deb,

Be sure to call the manufacturer of the tissue processor and ask if adding
cobalt chloride to the alcohol is permitted. Some chemicals may not be
acceptable for use  on one processor while the same chemicals may be
acceptable to use on a different processor.


Mark


MARK A. LEWIS


Product Specialist


Thermo Shandon


171 Industry Drive, Pittsburgh, PA 15275


Direct: (412) 747-4013


Fax: (412) 788-1097


e-mail: mark.lewis@thermoshandon.com


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                    "Van Eyck,                                             
                    Deb"                 To:                               
                                    
                                         cc:                               
                    01/02/02             Subject:                          
                    04:46 PM                                               
                                                                           
                                                                           




Hi all-
Does any one have the formula/recipe for a cobalt chloride solution to
color the final alcohol in the processor for small specimens or cell
blocks??? I've been told that this solution works better than adding eosin.
Please give me the product number for cobalt chloride ordered and MSDS of
final solution made in absolute alcohol. Also does anyone have any
experience using this on cell blocks made with histogel????  This product
makes an excellent cell block prep, but is very transparent and hard to see
when embedding and cutting. Any input would be appreciated.  Thanks!