From: | dgaupp@tulane.edu |
Hello Hisotnetters, Does anyone have a protocol on freezing tissue in liquid nitrogen? I know its usually best to fix the tissue, then cryoprotect it. I have a professor that just want to freeze the tissue without a fixative and cryoprotection? Also, I need a protocol on fixation/cryoprotection for another experiment. Any help would be appreciated.. Thanks, Dina