best method for freezing tissue?

From:dgaupp@tulane.edu

Hello Hisotnetters,

     Does anyone have a protocol on freezing tissue in liquid nitrogen?  I know 
its usually best to fix the tissue, then cryoprotect it.  I have a professor 
that just want to freeze the tissue without a fixative and cryoprotection?
         
     Also, I need a protocol on fixation/cryoprotection for another 
experiment.  Any help would be appreciated..


Thanks,

Dina   



     




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