Re: xylene substitutes

From:clocallo@leica-microsystems.com


Bruce,

The white milky look around the edges of the slides is due to the
incompatibility of the mountant with the substitute.  I have a list of
vendor mountants and substitutes that indicate what reagents are compatible
and what are not.  Please contact me for this information.  It may be able
to provide you with the information you need to be able to switch from
xylene to some substitutes.

Cathy Locallo B.A. HTL(ASCP)
Director Technical Assistance Center
Leica Microsystems
1 (800) 248-0123 x7051





Bruce Gapinski  on 12/26/2001 12:04:05 PM

To:   "'HistoNet@Pathology.swmed.edu'" 
cc:

Subject:  xylene substitutes


I need to know what xylene substitutes will work well w/ my Leica
auto-coverslipper. I'm in a temporary suite and the Dr. next door can smell
xylene. It's only about 7ppm but he has a right to complain.
     The thing I'm looking for is a clearing agent that smells like
nothing at all. Hemo-De won't do. Our Cyto department uses that, and it
would just make the office next door smell like a citrus grove.
     I have one gallon of something called Clear-Rite 3 by Richard Allen.
Smells ok but I'm getting a milky look around the edges of the slides as I
use it today.
     I must work fast as the Dr. is angry, and has threatened to "shut us
down if it doesn't smell better today".
     Please & Thank you.
 Bruce (I am not a rep) Gapinski HT (ASCP)

PS Happy Holidays


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