I have used normal serum from the species being stained as a way to absorb
secondary antibodies.
I block with serum before the primary antibody, before the secondary and
sometimes put some of the serum in the secondary antibody.
Patsy Ruegg

Njoydobro@aol.com wrote:

> Does anyone have any experience reducing background by absorbing secondary
> antibodies?
>
> Doug M.
> Ameripath