Re: best method for freezing tissue?

From:Nicole Cooper

I use both rat, mouse, human and ferret tissue which is fixed in 4% 
formaldehyde.  For cutting frozen sections I cryprotect the tissue in 30% 
sucrose/PBS overnight , then freeze the tissue onto the chuck with 
tissuetek.  This gives good morphology, but takes a little longer than the way 
you are currently doing it.
Hope this helps.
Nicole




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