Re: What Alcohol do you use? Ethanol vs. Methanol

From:Barry Rittman

1.    I cannot see no  logical reason for any change in staining.

2.    Methanol is a little faster than ethanol, is generally cheaper but is more toxic.

3.    Isopropyl alcohol is a slower dehydrating agent but is miscible with paraffin wax to a limited extent.
Barry

Histo-Scientific Research Laboratories wrote:

Dear Histonetters, We currently use a denatured, lab grade alcohol which consists of 95 parts ethyl and 5 parts isopropyl.  We are considering a switch to a mixture of 90 parts ethyl, 5 parts isopropyl and 5 parts methyl.  We will be using this reagent alcohol on our processor and stainer.  We are considering the switch for a simple reason:  easy pour bottles!  Would there be a noticeable difference in processing/staining due to the different makeup?  What is everyone else using? thanks, Beth PooleHSRL-A GLP Compliant Laboratory137 S. Main StreetWoodstock, VA  22664(540)459-8211fax: (540)459-8217beth@hsrl.orgwww.hsrl.org 

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