From: | Barry Rittman |
2. Methanol is a little faster than ethanol, is generally cheaper but is more toxic.
3. Isopropyl alcohol is a slower dehydrating agent
but is miscible with paraffin wax to a limited extent.
Barry
Histo-Scientific Research Laboratories wrote:
Dear Histonetters, We currently use a denatured, lab grade alcohol which consists of 95 parts ethyl and 5 parts isopropyl. We are considering a switch to a mixture of 90 parts ethyl, 5 parts isopropyl and 5 parts methyl. We will be using this reagent alcohol on our processor and stainer. We are considering the switch for a simple reason: easy pour bottles! Would there be a noticeable difference in processing/staining due to the different makeup? What is everyone else using? thanks, Beth PooleHSRL-A GLP Compliant Laboratory137 S. Main StreetWoodstock, VA 22664(540)459-8211fax: (540)459-8217beth@hsrl.orgwww.hsrl.org