Sorry for my admittably unclear text. We aren't using
coverplates for coverslips, of course. The problem I
mentioned occurs when applying normal coverslips AFTER
USING COVERPLATES FOR STAINING.

Thanks for your Coverplate hints!
I have ordered a few different aqueous mounting media
to give them a try. I hope they'll work better than
glycerin-gelatin.

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   Mikael Niku             URL: www.helsinki.fi/~mniku/
   University of Helsinki  Dept. Basic Veterinary Sciences
       - Mitäkö mieltä olen länsimaisesta sivistyksestä?
         Minusta se olisi erinomainen ajatus!
                                              - Gandhi
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----- Original Message -----
From: "Gayle Callis" 
To: 
Sent: Wednesday, January 09, 2002 6:27 PM
Subject: Shandon coverplates


> I have never seen the coverplates used like a coverslip.  The system is
> designed to hold a slide in such a way as to allow reagents, etc to added
> at the top for immunostaining, basically a gravity displacement/capillary
> gap system, then the coverplate is REMOVED at the end, the slide is
> coverslipped with an appropriate mounting media.
>
> Coverplates here are reused.  I usually remove the coverplate BEFORE
> chromogen step, in order to control color development, and that keeps
> chromogens from staining the polycarbonate plastic for another use. Care
is
> taken NOT to scratch the coverplates, if so, they are tossed.
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>