Andrew,
Try a different control.
You probably have an iron control that is so full of
iron, that it will set of a security alarm!! Try a
liver with mild siderosis, or possibly spleen.
 


--- Andrew Kennedy 
wrote: > Hi Histonetters,
> 
> A quality assurance survey that our lab participates
> in, tested us out on
> our Perl's Iron stain - the test sections were
> marked as satisfactory but
> our control section was deemed unsatisfactory due to
> a "diffusion of
> reaction product into the surrounding tissue,
> including nuclei". Does anyone
> have any ideas on how to fix this? Is it a problem
> with the stain or the
> tissue we use as a control?
> 
> Thanks in advance,
> 
> Andrew Kennedy
> 
> Senior Science Officer in charge - Histopathology.
> Department of Anatomical Pathology
> Concord Repatriation General Hospital
> Hospital Road
> Concord, NSW, Australia
> 2139
> Phone: +612 9767 6115
> Fax: +612 9767 8427
> 
> "Noli illegitimi carborundum"
> 
>
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=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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