Re: Mounting problem with Coverplated slides

From:Rose Richardson

Interesting! I have been having some difficulty with non-aqueous mounting
media and did not think of the coverplates as a possible problem. (drying
starting in the center of the mounted section after few weeks in a "fern"
type pattern)  I thought that the dehydration, xylene or the mounting media
itself was a problem and have changed and adjusted number of stations in the
dehydration, changed media and so on.  The problem has improved, but still
will happen on occasion.  I have noticed that it will happen with human
brain tissue but NOT the mouse brain ran on the same system as you have
(Shandon coverplate/non automated system).

Could any of the reps for Shandon give us feedback on any changes in the
Coverplates in the last few months?

Rose


----- Original Message -----
From: "Mikael Niku" 
To: "'Histonet'" 
Sent: Wednesday, January 09, 2002 6:09 AM
Subject: Mounting problem with Coverplated slides


Dear histonetters!

We recently started using Shandon Coverplates for
immunohistochemistry and in situ hybridization (without
the staining center or automatic stainer).

Everything seemed to be fine first and we were very happy
with the system. However, we soon noticed that using
Coverplates resulted in mounting problems. We are using
an aqueous mounting medium (traditional glycerin-gelatin
jelly) as the NBT/BCIP stain of in situ hyb seems to
fade with xylene-based stuff. This used to work just well,
but with Coverplated slides the mounting medium seems
to evaporate or contract somehow, and sections often
get dehydrated (and thus ruined). This happens very quickly,
in a matter of hours or days.

I can't understand why this might be. The slides don't
even go directly from Coverplates to mounting, but they
are at least washed a few times and counterstained and
again washed in between. But this really happens exactly
and only with slides that have been stained using Coverplates.

Any ideas, or better yet, suggestions for solutions?

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   Mikael Niku             URL: www.helsinki.fi/~mniku/
   University of Helsinki  Dept. Basic Veterinary Sciences
       - Mitäkö mieltä olen länsimaisesta sivistyksestä?
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                                              - Gandhi
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