From: | Lisa Dillard-Telm |
I've had several successful CD31 experiments on mouse tissue (formalin
fixed and
paraffin embedded) with PharMingen's antibody. Catalog # 550274
and
lot # M070846. I used it at a dilution of 1:500 overnight
in the refrigerator.
This protocol also needed amplification so I used the tyramide signal
amplification
kit from NEN.
Good luck
Lisa Dillard-Telm
mstarost@mail.mdanderson.org wrote:
Does anyone know of a source for CD31 that works on mouse tissue (formalin
fixed, paraf. embed.). DAKO's antibody for some reason does not work on mouse
or rat (ran it back to back with human and rhesus monkey tisssue , both of which
worked).Matthew Starost