Re: Daily Digest

From:Gayle Callis

Try an antibody directed specifically to mouse tissue, rat antiCD31
(PECAM-1) monoclonal from Pharmingen. They recommend trypsin digestion on
paraffin sections, we do our on acetone fixed frozens

At 08:46 AM 1/8/02 -0600, you wrote:
>
>
>Does anyone know of a source for CD31 that works on mouse tissue (formalin
>fixed, paraf. embed.).  DAKO's antibody for some reason does not work on
mouse
>or rat (ran it back to back with human and rhesus monkey tisssue , both of
which
>worked).
>
>Matthew Starost
>
>
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)





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