Dear Marsha et al,

Several companies, Zymed, DAKO and others now have a seperate, proprietary
reagent that you can add AFTER development of DAB.  DAKO calls theirs a DAB
enhancer and it works nicely for weakly stained antibodies.  The DAB will
turn a darker color than the usual brownish pigment.   Have used it with
their DAB+ and not with DAKo kits, but that's research folks.

It is not a tyramide product. 




At 08:30 AM 1/7/02 -0600, you wrote:
>Tim,
>Isn't this a signal amplification instead of an DAB Enhancer?  I have
>always used Copper Sulfate to enhance the DAB.
>
>Marsha
>
>On Mon, 07 Jan 2002 07:51:53 -0500 Tom McNemar 
>writes:
>> We use Ventana's Amplification step for a few antibodies.  It works 
>> very
>> well for us.  Info from the web site is below:
>>  
>> 
>> 
>> *	
>> 
>>      Amplification Kit Benefits 
>> 
>> *	Enhances signal to produce darker, easier to read stains 
>> 
>> *	Works with any primary anibody you are now using 
>> 
>> *	Reduces time required to read slides and make an accurate 
>> diagnosis 
>> 
>> *	May reduce the amount of antibody concentrate required 
>> 
>> *	Improves overall cost efficiency of the testing process by 
>> reducing
>> time to diagnosis 
>> 
>>      Product Order Codes
>> 
>>      760-080 Amplification Kit/
>>           NexES System
>>      250-080 Amplification Kit/
>>           ES System
>> 
>>  
>> 
>> Hope this helps,
>> Tom Mc Nemar HT(ASCP) 
>> Histology Supervisor 
>> Licking Memorial Hospital 
>> Newark, Ohio 
>> 
>> 
>>  
>>  -----Original Message-----
>> From: eileen dusek [mailto:eileen_dusek@hotmail.com]
>> Sent: Sunday, January 06, 2002 5:05 AM
>> To: histonet@pathology.swmed.edu
>> Subject: Dab Enhancement
>> 
>> 
>> Good Morning Everyone,
>> Does anyone know a procedure for DAB Enhancement on the Ventana.  I 
>> thought
>> many years ago they sold an enhancer which was in a dispenser.  I 
>> call
>> Ventana, they sent me a procedure using Nickel ,cobalt chloride. and 
>> DAB off
>> the machine. I am wondering if the nickel and cobalt chloride can be 
>> paced
>> on the tissue after the Ventana process has been completed or put in 
>> a
>> dispenser.
>> Any suggestion are greatly appreciated.
>> Eileen Dusek
>> Central DuPage Hospital
>> 
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>> 
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>> 
>> 
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Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

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