Tim,
Isn't this a signal amplification instead of an DAB Enhancer?  I have
always used Copper Sulfate to enhance the DAB.

Marsha

On Mon, 07 Jan 2002 07:51:53 -0500 Tom McNemar 
writes:
> We use Ventana's Amplification step for a few antibodies.  It works 
> very
> well for us.  Info from the web site is below:
>  
> 
> 
> *	
> 
>      Amplification Kit Benefits 
> 
> *	Enhances signal to produce darker, easier to read stains 
> 
> *	Works with any primary anibody you are now using 
> 
> *	Reduces time required to read slides and make an accurate 
> diagnosis 
> 
> *	May reduce the amount of antibody concentrate required 
> 
> *	Improves overall cost efficiency of the testing process by 
> reducing
> time to diagnosis 
> 
>      Product Order Codes
> 
>      760-080 Amplification Kit/
>           NexES System
>      250-080 Amplification Kit/
>           ES System
> 
>  
> 
> Hope this helps,
> Tom Mc Nemar HT(ASCP) 
> Histology Supervisor 
> Licking Memorial Hospital 
> Newark, Ohio 
> 
> 
>  
>  -----Original Message-----
> From: eileen dusek [mailto:eileen_dusek@hotmail.com]
> Sent: Sunday, January 06, 2002 5:05 AM
> To: histonet@pathology.swmed.edu
> Subject: Dab Enhancement
> 
> 
> Good Morning Everyone,
> Does anyone know a procedure for DAB Enhancement on the Ventana.  I 
> thought
> many years ago they sold an enhancer which was in a dispenser.  I 
> call
> Ventana, they sent me a procedure using Nickel ,cobalt chloride. and 
> DAB off
> the machine. I am wondering if the nickel and cobalt chloride can be 
> paced
> on the tissue after the Ventana process has been completed or put in 
> a
> dispenser.
> Any suggestion are greatly appreciated.
> Eileen Dusek
> Central DuPage Hospital
> 
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