This has been our experience as well.
1)Filter the carbol fuchsin before each use (and you can use it over and
over again).
2)Use bottled Distilled water.
3)Use adhesive slides to provide better bonding with the tissue through the
various staining steps.
We routinely use coplin jars for this stain and have not had to resort to
flat racks to prevent "jumping"

Rajan Bawa, Ph.D
Colorado Histo-Prep
(970)493-2660
www.histoprep.net
www.histoprep.com


----- Original Message -----
From: "Weems, Joyce" 
To: "'Amos Brooks'" ; 
Sent: Thursday, January 03, 2002 2:23 PM
Subject: RE: AFB's


> My guess also. And be sure to filter it before each use no matter how long
> you use it!
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
>
>
> -----Original Message-----
> From: Amos Brooks [SMTP:amosbrooks@home.com]
> Sent: Thursday, January 03, 2002 3:03 PM
> To: histonet@pathology.swmed.edu
> Subject: Re: AFB's
>
> Ok,
>     Maybe this is a dumb question, but do you reuse your carbol
> fuchesin, and if you do, how often do you filter or dump it? It is
feasible
> that the solution itself is contaminated and this could be getting on the
> slides. Also make sure the slides are washed ** well ** in water before
> differentiating (the water will not bleach out the carbol fuchesin so let
it
> run). I have seen the dye start to precipitate and if this gets on the
> slides sometimes it resembles a bug.
>     See to it also that the waterbath is clean during sectioning
> too. I have heard of this causing problems too. If you're doing all this
you
> should be fine and for the moment I'm out of ideas.
> Amos Brooks
>
> ----- Original Message -----
> From: Noreen Gilman 
> To: histonet@pathology.swmed.edu
> 
> Sent: Thursday, January 03, 2002 1:49 PM
> Subject: AFB's
>
> Hello everyone in histoland.  I've got an unusual problem
> and I hope you guys can help me.
> My pathologist claims that when we do an AFB, some of the
> bacillus from the positive control "jumps" onto the patient slide and
> contaminates it. He claims he sees stray bacillus on the slides
surrounding
> the control tissue, and he thinks he see them on the patient slide as
well.
> I have tried several ways of doing this stain and at first thought the
> tubing on our sink was contaminated. We changed the tubing and used only
> distilled water, and he still insists they are there.  I have never had a
> problem like this before. I'm at my wits end. Any suggestion are very
> welcome.
> Noreen
>
> Noreen S. Gilman, HT (ASCP) CLS
> Broward General Medical Center
> Ft. Lauderdale, FL 3316
> 954-355-5592 Phone
> 954-355-4139 Fax
> ngilman@nbhd.org 
>
>
>