We stain our slides on a rack with the control tissue in a separate block,  
and don't have any problems with "jumping bacilli" in our ZN technique.  
However, we have had problems with our Auramine Rhodamine technique which 
uses a coplin jar.  We have had a few cases where the original sections 
contained a few stray bacilli, but after re-making and/or filtering the 
solution the sections were negative.  We now make a point of always 
filtering the solution before it's used and do both staining methods on 
consecutive sections.

Rachel

>From: Barbara Stancel 
>To: Ngilman@nbhd.org, histonet@pathology.swmed.edu
>Subject: Re: AFB's/ruling out jumping bacilli
>Date: Fri, 04 Jan 2002 02:05:51 +0000
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>From histonet@pathology.swmed.edu Thu, 03 Jan 2002 18:37:47 -0800
>Message-id: 
>
>
>Dear Noreen,    We also have a pathologist who talked about "jumping
>bacilli."  We changed our staining procedure and now perform our AFBs the
>same way Andi described: we stain the slides flat, wash them individually,
>and always use fresh dye--filtering only the amount we need just before
>staining.  Not one single complaint since we changed!
>
>Barbara
>
>USDA, FSIS, OPHS, Eastern Laboratory, Pathology
>RRC, 950 College Station Road
>Athens, Georgia 30604
>phone: (706) 546-3556
>fax: (706) 546-3589
>
>
>
>>From: Andrea Grantham To: Noreen Gilman , histonet@pathology.swmed.edu
>>Subject: Re: AFB's/ruling out jumping bacilli Date: Thu, 03 Jan 2002
>>14:08:59 -0700
>>
>>Noreen, I had a pathologist who drove me nuts with the same idea. Instead
>>of staining in a coplin jar I laid the slides down on a staining rack and
>>stained them flat so that the stains or wash fluids were used only for one
>>slide. Also use fresh stains. This way those nasty bacteria can not jump
>>from slide to slide, or they can't be swimming around in the coplin jars
>>getting ready to jump on any unsuspecting patient tissue. Andi
>>
>>
>>
>>At 01:49 PM 1/3/02 -0500, you wrote:
>>>Hello everyone in histoland. I've got an unusual problem and I hope you
>>>guys can help me. My pathologist claims that when we do an AFB, some of
>>>the bacillus from the positive control "jumps" onto the patient slide and
>>>contaminates it. He claims he sees stray bacillus on the slides
>>>surrounding the control tissue, and he thinks he see them on the patient
>>>slide as well. I have tried several ways of doing this stain and at first
>>>thought the tubing on our sink was contaminated. We changed the tubing 
>>>and
>>>used only distilled water, and he still insists they are there. I have
>>>never had a problem like this before. I'm at my wits end. Any suggestion
>>>are very welcome. Noreen
>>>
>>>Noreen S. Gilman, HT (ASCP) CLS Broward General Medical Center Ft.
>>>Lauderdale, FL 3316 954-355-5592 Phone 954-355-4139 Fax ngilman@nbhd.org
>>
>>..................................................................... :
>>Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research
>>Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 :
>>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097)
>>(email: algranth@u.arizona.edu) :
>>:...................................................................:
>>http://www.cba.arizona.edu/histology-lab.html
>>
>>
>
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