Point well taken and recorded.  

Since I was always looking for extravascular accumulations of RBC's the
"upside of the downside" was quite acceptable.  I got to the point at which
I would throw a N.D. filter into the mix to find RBC's, remove it to quench
the hole (marker) then reinsert it to find the next.  At the end, I would
switch to BF and low power, take a digital and have an image/record that
could be analyzed.

Thanks,

Fred


> ----------
> From: 	Barry Rittman
> Sent: 	Thursday, January 10, 2002 2:28 PM
> To: 	histology
> Subject: 	Re: vital stain
> 
> Frederick
> Thank you for this excellent suggestion.
> It is true that the eosin stain will fade with the correct "fluorescence"
> illumination.  I would recommend that the section be examined by indirect
> (epi-)
> illumination. The level of light intensity that is required for such
> illumination is significantly lower than for transillumination. This
> results in
> a much slower rate of fading of the eosin.
> I routinely examine old H and E stained section with this and the eosin is
> much
> more brilliant than when examined by routine brightfield microscopy..
> An additional advantage is that  there is improved  resolution.
> Of course not everyone has the availability of epi- illumination for their
> microscope.
> Barry
> 
> "Monson, Frederick C." wrote:
> 
> > Morning,
> >         Just on the chance that this would be appropriate for your
> effort, I
> > will make a suggestion for a simple, not quite-non-destructive, method
> for
> > spotting early capillary damage in routine specimens.
> >         Background:  Fresh Eosin preparations have a green sheen when
> > exposed to the UV in daylight or even fluorescent illumination.  FRESH!
> >
> >         Utility:  As a consequence of the eosinophilia of RBC's, one can
> use
> > any fluorescent scope with a FITC cube or filters to demonstrate the
> > distribution of RBC's in any H&E section.
> >
> >         Downside:  After exposure to UV the eosin background will have a
> > visible hole in it when viewed with normal brightfield illumination.
> >
> >         Upside of Downside:  One can always relocate those areas on
> which
> > one paused 'for a look' while using the UV.
> >
> > Hope this helps,
> >
> > Fred Monson
> >
> > Frederick C. Monson, PhD
> > Center for Advanced Scientific Imaging
> > West Chester University
> > West Chester, Pennsylvania, USA, 19383
> > 610-738-0437
> > fmonson@wcupa.edu
> >
> > > ----------
> > > From:         Zubovits, Dr. Judit
> > > Sent:         Wednesday, January 9, 2002 7:19 PM
> > > To:   'histonet@pathology.swmed.edu'
> > > Subject:      vital stain
> > >
> > > Hello Histonet-ters;
> > >
> > > I wonder if you could help me with the following:
> > >
> > > I am looking at "muscle biopsies" from volunteers (mice) who have
> received
> > > focused ultrasound treatment.  Of course enough excess heat would cook
> any
> > > tissue, but I we are trying to detect much more subtle changes/damage
> > > produced by a lot less heat.
> > >
> > > I know TTC has been used on hearts to detect ischemic necrosis
> associated
> > > with myocardial infarction, but as I understand it, you need to soak
> the
> > > tissue *before* paraffin embedding, and TTC is intended to show areas
> of
> > > necrosis at the gross, not microscopic level.
> > >
> > > Is there anything out there for the microscopic level that would
> highlight
> > > areas of damage?
> > >
> > > Thanks for all your help.
> > >
> > > Judit Zubovits
> > >
> > >
> 
> 
>