Morning,
	Just on the chance that this would be appropriate for your effort, I
will make a suggestion for a simple, not quite-non-destructive, method for
spotting early capillary damage in routine specimens.  
	Background:  Fresh Eosin preparations have a green sheen when
exposed to the UV in daylight or even fluorescent illumination.  FRESH!

	Utility:  As a consequence of the eosinophilia of RBC's, one can use
any fluorescent scope with a FITC cube or filters to demonstrate the
distribution of RBC's in any H&E section.

	Downside:  After exposure to UV the eosin background will have a
visible hole in it when viewed with normal brightfield illumination.

	Upside of Downside:  One can always relocate those areas on which
one paused 'for a look' while using the UV.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson@wcupa.edu


> ----------
> From: 	Zubovits, Dr. Judit
> Sent: 	Wednesday, January 9, 2002 7:19 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	vital stain
> 
> Hello Histonet-ters;
> 
> I wonder if you could help me with the following:
> 
> I am looking at "muscle biopsies" from volunteers (mice) who have received
> focused ultrasound treatment.  Of course enough excess heat would cook any
> tissue, but I we are trying to detect much more subtle changes/damage
> produced by a lot less heat.  
> 
> I know TTC has been used on hearts to detect ischemic necrosis associated
> with myocardial infarction, but as I understand it, you need to soak the
> tissue *before* paraffin embedding, and TTC is intended to show areas of
> necrosis at the gross, not microscopic level.
> 
> Is there anything out there for the microscopic level that would highlight
> areas of damage?
> 
> Thanks for all your help.
> 
> Judit Zubovits
> 
>