Hi,

I use a Mayer's hematoxylin and really like it. I have made it in one day if
I was in a hurry. All you need to do is stir it till it is completely
dissolved at each step. I didn't see any staining differences if I made it
in one day or three. 

Good luck

Jennifer Harvey
University of Rochester Medical Center
Department of Orthopaedics
601 Elmwood Ave Box 626
Rochester,  NY 14642
585-273-4129



Mayer's Acid Hemalum Modified by Lillie 

Hematoxylin C.I. 75290 (Sigma
H-3136)---------------------------------------2.5 gm
Distilled
Water-----------------------------------------------------------------------
350 ml 
Aluminum Ammonium Sulfate (Sigma A-2140)---------------------------------25
gm
Sodium Iodate (Sigma
S-4007)--------------------------------------------------0.25 gm
Glycerol (VWR
IB15762)---------------------------------------------------------150 ml
Glacial Acetic
Acid-------------------------------------------------------------------10 ml

Solution Preparation:

Dissolve 2.5 gm Hematoxylin by holding overnight in 350 ml distilled water.
Next day add 25 gm Aluminum Ammonium Sulfate and 0.25 gm Sodium Iodate and
let sit overnight. Day 3 add 150 ml Glycerol and 10 ml Glacial Acetic Acid.
After filtering this can be used immediately. Use for 1-2 weeks. Stock
stable 4-6 weeks.


> ----------
> From: 	LOUISE YARROW
> Sent: 	Friday, January 4, 2002 6:34 PM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	mayers heamatoxlyn
> 
> I have tried to order chloral hydrate to make mayers but have found out
> it is a conrtrolled chemical and not available in Canada any longer. 
> Can anyone tell me how they make mayers without chloral hydrate, or is
> that possible?
> We have been using it in our cytology dept.
> Thanks for any suggestions
> Louise Yarrow
> CLS
> Foothills hospital
> Calgary, Alberta
>