Robert
A protocol I have used successfully on gross specimens, although not for
lipid plaques in major blood vessels, is as follows:
Thoroughly fix the specimen.
Thoroughly wash the specimen in running tap water - depends upon the size of
the specimen but I would suggest at least 2 hours.
Place in a Sudan III solution (0.2g Sudan III, 70 ml absolute ethanol, 30 ml
distilled water) until the fat is well stained. (Should not need more than 2
hours unless the fatty area is huge).
Wash in 70% ethanol to remove excess stain.
Transfer to 10% neutral buffered formalin.
The specimen can be mounted in a plastic museum pot in NBF, if required.
Regards
Roy Ellis
mailto:roy.ellis@imvs.sa.gov.au


> -----Original Message-----
> From: Robert Geske [mailto:RGeske@lexgen.com]
> Sent: Friday, 11 January 2002 04:41 AM
> To: histonet@pathology.swmed.edu
> Subject: en face staining of aorta
>
>
> Does anyone have a protocol available for the staining of lipid plaques in
> major blood vessels?  this would be on the intact vessel that had been
> fixed, opened along the long axis and pinned --- the staining
> being done on
> the gross specimen.  Sudan IV, ORO, or other suggestions are welcome.
>
>
> thanks in advace,
> rob
>
>
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