Rachel,
I prepare my own Gill's Hematoxylin (I don't like commercially prepared
dyes) and have found that it just gets better with age (to a point) and it
doesn't have the mercuric oxide in it.  

Connie McManus


At 01:14 PM 12/19/01 +0100, Van Hecke Donald wrote:
>Rachel,
>
>I had the same problem concerning the Mercuric oxide in the Harris's
>haematoxylin and personal safety/waste disposal. I changed my Haematoxylin
>to a Gill II and use it for Papanicolaou and H&E staining (both regressive
>staining with short differentiation in weak acid alcohol). I am very
>satisfied with it. If you would like to make the staining solutions
>yourself, I can advise you to look into the Stainfile webpages. This website
>has some exellent pages on Haematoxylin, including recipies for home
>brewing. You can find the stainfile at : http://stainsfile.info . Success!
>
>Best regards,
>
>Donald Van Hecke
>Lab. Ana. Path.
>AZ St. Lucas - Brugge
>Belgium
>
>
>
>> -----Oorspronkelijk bericht-----
>> Van:	Rachel Stoat [SMTP:r_stoat@hotmail.com]
>> Verzonden:	woensdag 19 december 2001 11:31
>> Aan:	HistoNet@pathology.swmed.edu
>> Onderwerp:	Haematoxylin
>> 
>> For health and safety reasons I am looking for a replacement for Harris's 
>> Haematoxylin (or at least the mecuric oxide in it) which we use on a 
>> linistain.  I would prefer not to buy a ready made solution, and the
>> results 
>> need to be similar to what we are getting now.
>> Any suggestions or references would be appreciated!
>> 
>> Thanks!
>> Rachel
>> 
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>

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