I have never come across rehydration using glycerol. (What does it do?)

Using normal (physiological(0.9%)) saline, immersion for 60 secs (some say less) works very well. 
After "time's up", remove the smear, flick the saline off and immediately wet fix. It works well *and* gets rid of blood.

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire
terry.marshall@rgh-tr.trent.nhs.uk

-----Original Message-----
From: p=NHS NATIONAL INT;a=NHS;c=GB;dda:RFC-822=kb2drkprk(a)yahoo.com; 
Sent: 04 January 2002 22:36
To: p=NHS NATIONAL
INT;a=NHS;c=GB;dda:RFC-822=histonet(a)pathology.swmed.edu;
Subject: Glycerol rehydration of FNA's


Does anyone have a procedure for rehydrating air-dried
Fine Needle Aspirates using glycerol?  In the Histonet
Archives, it was mentioned that a 50% glycerol
solution could be used to rehydrate cells so that they
regain their dye penetrablility for Pap staining.  How
long should the air-dried slides be left in the
glycerol solution in order to rehydrate them?

Thanks!

Kelly Booher, HTL
Anatomic Pathology
Swedish Medical Center, Providence Campus
Seattle, WA  98122


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