Either 5 % formic acid (aq) or EDTA decal will work without deliterious
effects.
The thing that is tricky is whether the GFP chosen is stable to organic
solvents (e.g. alcohol) or not. EGFP from Clontech or any of the enhanced
variants are alcohol stable. See their Living Colors booklet at
www.clontech.com

Donna Montague, M.S.
Research Associate
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
(501) 603-1239

-----Original Message-----
From: rueggp [mailto:rueggp@earthlink.net]
Sent: Wednesday, January 02, 2002 3:04 PM
To: Johnson, Teri
Cc: Histonet (E-mail)
Subject: Re: GFP


i bet it does depending on what type of decal solution and for how long.
patsy ruegg

"Johnson, Teri" wrote:

> Does anyone out there know if decalcification affects GFP positive bones?
>
> Teri Johnson
> Manager Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110
> tjj@stowers-institute.org