My guess also. And be sure to filter it before each use no matter how long
you use it!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Amos Brooks [SMTP:amosbrooks@home.com]
	Sent:	Thursday, January 03, 2002 3:03 PM
	To:	histonet@pathology.swmed.edu
	Subject:	Re: AFB's

	Ok,
	    Maybe this is a dumb question, but do you reuse your carbol
fuchesin, and if you do, how often do you filter or dump it? It is feasible
that the solution itself is contaminated and this could be getting on the
slides. Also make sure the slides are washed ** well ** in water before
differentiating (the water will not bleach out the carbol fuchesin so let it
run). I have seen the dye start to precipitate and if this gets on the
slides sometimes it resembles a bug.
	    See to it also that the waterbath is clean during sectioning
too. I have heard of this causing problems too. If you're doing all this you
should be fine and for the moment I'm out of ideas.
	Amos Brooks

		----- Original Message ----- 
		From: Noreen Gilman   
		To: histonet@pathology.swmed.edu
  
		Sent: Thursday, January 03, 2002 1:49 PM
		Subject: AFB's

		Hello everyone in histoland.  I've got an unusual problem
and I hope you guys can help me.
		My pathologist claims that when we do an AFB, some of the
bacillus from the positive control "jumps" onto the patient slide and
contaminates it. He claims he sees stray bacillus on the slides surrounding
the control tissue, and he thinks he see them on the patient slide as well.
I have tried several ways of doing this stain and at first thought the
tubing on our sink was contaminated. We changed the tubing and used only
distilled water, and he still insists they are there.  I have never had a
problem like this before. I'm at my wits end. Any suggestion are very
welcome.
		Noreen
		 
		Noreen S. Gilman, HT (ASCP) CLS
		Broward General Medical Center
		Ft. Lauderdale, FL 3316
		954-355-5592 Phone
		954-355-4139 Fax
		ngilman@nbhd.org