RE: AFB's

From:Cynthia Favara

I have two immediate thoughts.
 
    1. Have you seen the slides to see what the problem is.
    2. Could be a problem with under fixed control tissue. If the control slide is cut at the same time as the specimen possible floaters would remain in the water bath and make it onto the patient slide.
 
Just two thoughts.
 

Cynthia Favara
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
406-363-9317
FAX 406-363-9286

-----Original Message-----
From: Noreen Gilman [mailto:Ngilman@nbhd.org]
Sent: Thursday, January 03, 2002 11:50 AM
To: histonet@pathology.swmed.edu
Subject: AFB's

Hello everyone in histoland.  I've got an unusual problem and I hope you guys can help me.
My pathologist claims that when we do an AFB, some of the bacillus from the positive control "jumps" onto the patient slide and contaminates it. He claims he sees stray bacillus on the slides surrounding the control tissue, and he thinks he see them on the patient slide as well. I have tried several ways of doing this stain and at first thought the tubing on our sink was contaminated. We changed the tubing and used only distilled water, and he still insists they are there.  I have never had a problem like this before. I'm at my wits end. Any suggestion are very welcome.
Noreen
 
Noreen S. Gilman, HT (ASCP) CLS
Broward General Medical Center
Ft. Lauderdale, FL 3316
954-355-5592 Phone
954-355-4139 Fax
ngilman@nbhd.org

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