Hello

I am looking for a staining technique to differentiate between living and dead root 
tissue (preferably highlighting the dead tissue) in onion seedlings infected with 
Sclerotium cepivorum. I have dabbled with neutral red (Jensens stain), Phloroglucinol, 
and Fluoroscien diactetate without great success. Does anybody have any other stains 
and staining techniques (or improvements on the techniques I am currrently trying) 
they can suggest? The end purpose of this staining procedure is to improve the 
resolution between living and dead tissue for digital image analysis. Thank you in 
advance for your help.

Kind regards

Sarah


Sarah A. Hunger M.Sc.
Soil, Plant and Ecological Sciences Division
PO Box 84 
Lincoln University
Canterbury
Phone: +64 3 325 2811 x 8157
Mobile: +64 21 899 420
Email: hungers@lincoln.ac.nz