Glycerol rehydration of FNA's

From:Kelly Booher

Does anyone have a procedure for rehydrating air-dried
Fine Needle Aspirates using glycerol?  In the Histonet
Archives, it was mentioned that a 50% glycerol
solution could be used to rehydrate cells so that they
regain their dye penetrablility for Pap staining.  How
long should the air-dried slides be left in the
glycerol solution in order to rehydrate them?

Thanks!

Kelly Booher, HTL
Anatomic Pathology
Swedish Medical Center, Providence Campus
Seattle, WA  98122


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