Fwd: Re: Cobalt Chloride
Vinnie wanted me to clarify where I use the cobalt chloride on my
processor. Actually I put it in the first absolute ETOH. When that starts
to change color from blue to pink I dump it and rotate the alcohols up
always having the last absolute be the freshest. This is sort of my
"reagent management system". Once the tissues take on the blue stain on the
outside it only goes away in the presence of water and the remaining
stations on the processor are hopefully free of water. If you put the
cobalt chloride in the last ETOH (or if you use too much)it carries over to
the xylenes and you have bluish xylenes which I guess is OK (my processor
is a older tissue tek dipper not an enclosed processor). I just like for
any excess to be washed off in the other ETOH stations.
Andi
>X-Mailer: Novell GroupWise Internet Agent 6.0
>Date: Fri, 04 Jan 2002 09:33:51 -0500
>From: "Vinnie Della Speranza"
>To:
>Subject: Re: Cobalt Chloride
>
>Hi Andi,
>you didn't state where on the process you put your cobalt chloride. I
>assume the last absolute alcohol but thought you might want to clarify for
>histonet.
>regards,
>Vinnie
>
> >>> Andrea Grantham 01/03/02 04:16PM >>>
>I use cobalt chloride on my old tissue tek to help me to determine when the
>100% ETOH stations need to be changed since I do not process daily. I have
>also used it in an enclosed processor without any problems. If you use the
>right amount you get a blue stain on the tissue that makes the embedding so
>much easier. We loved using this for small gastric biopsies because you
>could differentiate the mucosal layer without any problem - cervical
>biopsies too. If you use too much the whole tissue stains blue making it a
>little harder to see the different layers of tissue. In the dip'n dunker I
>have now I use about a half teaspoon/1000ml.
>When you cut your section and float it on the waterbath the blue color goes
>away.
>Andi
>.....................................................................
>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
>: Sr. Research Specialist University of Arizona :
>: (office: AHSC 4212) P.O. Box 245044 :
>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
>: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
>:...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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