> -----Original Message-----
> From:	Mack, Jacquie 
> Sent:	Tuesday, January 08, 2002 7:43 AM
> To:	'Histonet@pathology.swmed.edu '
> Subject:	H&E artifact
> 
> Hello everyone!
> 
> We are again having some problems with our H&E stain.  There is some very
> inconsistent artifact appearing on some slides.  We are suspecting it may
> be our paraffin wax not being completely removed. (any other ideas?).  We
> use Paraplast wax.
> 
> I am wondering how often other labs are changing the alcohols and xylenes
> on their automated stainer? After how many runs / baskets / slides?  We
> have a Sakura Tissue Tec DRS stainer.
> 
> Also, is anyone using recycled solvents? 
> 
> Thanks in advance!
> 
> Jacquie Mack
> Tech II Main Lab
> Histopathology FMC
> Phone: 670-1574
> Fax: 270-4093
>