Advanced bone techniques
From: | "Renton, Lousie, Mrs" <177louie@chiron.wits.ac.za> |
Hi there,
3 Questions:
1. Anyone out there ever done PCR (polymerase chain reaction) or
in-situ hybridization of on either glycol or methyl methacrylate
sections?
2. would frozen sections of bone be suitable for the above
techniques, and if so, would the target DNA/RNA be destroyed by
cryopreservation?
3.Would optimal sections only be obtained with some sort of tape
transfer method or are there other techniques that could be used?
TIA
Best regards
Louise Renton
Bone research unit
MRC
Johannesburg
South Africa
<< Previous Message | Next Message >>