flotation of bone(tissue) on slides
Hi Histonetters,
I need some advice on decalcification of mouse leg (1cm x1mm). I let the
tissue fix in a decalcifier called "Cal-Rite" by Richard Allan Scientific for
over the weekend. Cal-Rite is a fixative and decalifier in one. The bone felt
like rubber. I then cut the leg into several little pieces(1 mm x 1 mm). When
I cut the tissue on the microtome(paraffin-embedded), it was very hard . So I
surface decal. for 30 minutes. I went to go cut, it was fine. But several
slides down the line, I had to surface again and again. My slides came out
really bad. The tissue was floating everywhere. I used gelatin in my water
bath and superfrost slides(Fisher). Would it make a difference if I
use "superfrost +" plus slides?
Any help would be greatly appreciated.
Thanks for your time,
Dina D. Gaupp, MT
Tulane Medical School
Center for Gene Therapy
email: dgaupp@tulane.edu
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