Happy Chinese New Year to all in Histoland.

I am doing fresh frozen sections of muscle and am getting freezing artifact.

My protocol is to remove the muscle and embed in OCT in a CO2/ methylbutane
slurry.  Then I section at -18, fix the sections, and b-Gal stain.  My
question is this: can I cryo-protect with sucrose prior to
embedding/freezing without fixing the muscle first?  Would the sucrose
interfere with staining?

Thanks (again and again and again).
Jen



Jennifer M. Philopena
Scientist 1
Canji, Inc.
3525 John Hopkins Court
San Diego, CA 92121
jennifer.philopena@canji.com