|From:||"Philopena, Jennifer" <firstname.lastname@example.org>|
Happy Chinese New Year to all in Histoland.
I am doing fresh frozen sections of muscle and am getting freezing artifact.
My protocol is to remove the muscle and embed in OCT in a CO2/ methylbutane
slurry. Then I section at -18, fix the sections, and b-Gal stain. My
question is this: can I cryo-protect with sucrose prior to
embedding/freezing without fixing the muscle first? Would the sucrose
interfere with staining?
Thanks (again and again and again).
Jennifer M. Philopena
3525 John Hopkins Court
San Diego, CA 92121
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