adhesion problems

From:Jason Bartz <>

<!doctype html public "-//W3C//DTD W3 HTML//EN"> <html><head><style type="text/css"><!-- blockquote, dl, ul, ol, li { margin-top: 0 ; margin-bottom: 0 } --></style><title>adhesion problems</title></head><body> <div> <font face="Times" color="#000000">I have been having some difficulty in getting sections to adhere to slides when cutting certain blocks.  I am working with phosphate buffered formalin fixed paraffin embedded hamster brain and have tested several slide drying times and temperatures as well as different slides (i.e. uncoated, gelatin coated and positively charged) on blocks that have a tendency for the sections to lift.  Changing these variables did not seem to help.  What I have noticed is after drying the sections slight bubbles can be seen between the section and the slide on some of the sections.  Of course, the sections that had the "bubbles" lifted from the slide while the sections that seemed to be in full contact with the slide (by visual inspection) looked fine.  I am new at histology and was wondering what is causing this an how can I reduce this phenomenon?  Thank you.<br> <br> Jason</font><br> <font face="Times" color="#000000"></font></div> <div>-- <br> ___________________________________________________<br> Jason C. Bartz, Ph.D.<br> Department of Medical Microbiology and Immunology<br> Creighton University<br> 2500 California Plaza<br> Omaha, NE 68178<br> (712) 280-1811 voice<br> (712) 280-1875 fax<br> </div> </body> </html>
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