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<div> <font face="Times" color="#000000">I have been having some
difficulty in getting sections to adhere to slides when cutting
certain blocks. I am working with phosphate buffered formalin
fixed paraffin embedded hamster brain and have tested several slide
drying times and temperatures as well as different slides (i.e.
uncoated, gelatin coated and positively charged) on blocks that have a
tendency for the sections to lift. Changing these variables did
not seem to help. What I have noticed is after drying the
sections slight bubbles can be seen between the section and the slide
on some of the sections. Of course, the sections that had the
"bubbles" lifted from the slide while the sections that seemed to be
in full contact with the slide (by visual inspection) looked fine.
I am new at histology and was wondering what is causing this an how
can I reduce this phenomenon? Thank you.<br>
<br>
Jason</font><br>
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<div>-- <br>
___________________________________________________<br>
Jason C. Bartz, Ph.D.<br>
Department of Medical Microbiology and Immunology<br>
Creighton University<br>
2500 California Plaza<br>
Omaha, NE 68178<br>
(712) 280-1811 voice<br>
(712) 280-1875 fax<br>
jbartz@creighton.edu </div>
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