Vacuoles Artifacts

From:Beckers <>

Help please Histonetters,

Occasionally experiencing small vacuoles in the epidermis of skin processed
for Mohs.  Today since the Dr. was processing a small melanoma case it was
esp. aggravating. Any ideas on what causes this and how to rid the finished
product of this confusing artifact??  I do frozen sections in -22 C to -25 C
cryostat chamber-have never used liquid Nitrogen and probably won't start
now.  Do have access to a -45 C cold bar in a second cryostat-doesn't appear
to be a staining phenomena but more a processing fluke.  Any ideas will be
greatly appreciated.

Sue Becker, HTL
Albany, NY

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