Trichrome for Fresh Frozen sections

From:"Philopena, Jennifer" <>

Thank you to all who responded to my problem with skin processing.  The
insight was so helpful.

Now do any of you have a procedure to stain fresh frozen sections with
Masson's Trichrome?  We're not sure how to fix the tissue prior to staining.
With FFPE tissue we de-paraffin, re-hydrate, and then plunk into warm Bouins
for 60 minutes.  I was thinking we could just go right into Bouin's with the
frozen sections.

Thanks in advance.


Jennifer M. Philopena
Scientist 1
Canji, Inc.
3525 John Hopkins Court
San Diego, CA 92121

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