>Hi all,
>
>Recently we have been having terrible terrible problems with tissues
>falling off the slides during HIER.  We (like most people) have had
>intermittant problems in the past with specific cases, but this is now a
>generalised problem.  We coat our own silanised slides, and have tried
>making up new batches of these.  We have also tried purchasing commercial
>polysine slides which were as bad or worse.  We curretly use MW HIER - is
>a pressure cooker any better for keeping tissues on slides?  Any other
>suggestions?  I'm rapidly running out of hair....
>
>Many thanks,
>Aidan, New Zealand.
>
>
>_____________________________________
>a i d a n   c   s c h u r r
> section head, histology
>  department of pathology
>hutt valley health
>high street, lower hutt
>wellington
>new zealand
>telephone ++64 4 5709173
>facsimile ++64 4 5709214

************

Hi Aidan,

We have been facing the same problem with the fatty parts of
atherosclerotic tissues. After mounting the paraffin sections on silan
coated glass slides, we keep them in the stove at 37°C for at least 4 days
before staining. We perform HIER in citrate buffer (15 min boiling, 10 min
cool-down) in an ordinary house hold microwave oven.
Keep you hair on!!!

Chris van der Loos
Dept. Cardiovascular Pathology
Academical Medical Centre H0-120
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands