Dear Deb:

Zymed offers two E-cadherin mouse monoclonal antibodies for FFPE tissue
staining (clones: 4A2C7 and HECD-1). Both require HIER with citrate buffer. We
also offer the BCA-225 antibody (clone: CU-18) which works well without
antigen recovery.

Sincerely,

John

--

"Van Eyck, Deb" wrote:

>
> Hi all,
>
>     ? for all those using e-cadherin on breast cases-- does this stain
> negative
> breast tissue - how do you find the antibody to work with?
>
> How about BCA-225-CU18- How many are routinely using this antibody and how
> is it to work with -- what dilutions? kits? target retrieval etc.
>
> Same for polyclonal CD117- ckit.
>
> Any help or tips on these new antibodies would help in working them up.  I
> will be glad to share with you all.  Thanks Deb