Date sent: Wed, 24 Jan 2001 17:23:59 -0500
From: "Philopena, Jennifer" <email@example.com>
To: "'Histonet'" <firstname.lastname@example.org>
> Happy Chinese New Year to all in Histoland.
> I am doing fresh frozen sections of muscle and am getting freezing artifact.
Jen, I would avoid any form of "cryo-protection" with muscle as this
tends to provide the tissue with enough time to develop ice crystal
artefact before freezing (producing those nasty little holes within
the fibre). Expose the fibres as best you can (small block) and
immerse in pre cooled (LN2) isopentane, preferably on the cryostat
chuck (specimen holder). If you attach the tissue to the chuck after
freezing avoid any thawing and re-freezing as this will introduce
even worse artefact.
The above observations are the result of personal experience and a
number of frustrating hours attempting to get perfectly preserved
Best of luck/skill,
Medical Research Council,
Oxfordshire, OX11 ORD
01235 834393 x360
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